Pharmacology & Physiology

Research Methods

Electrophysiology:

Whole-cell patch clamping in mammalian cells

Two-electrode voltage clamp in Xenopus oocytes

Excised inside-out patch clamp in Xenopus oocytes

Extracellular recordings on skin nerve preparation

Planar lipid bilayers

Fluorescence

Calcium imaging, dual excitation with fura-2

Fluorescence resonance energy transfer (FRET)

Molecular Biology

Transfection of mammalian cells

Mutation of cDNA to study recombinant ion channels

RNA preparation and injection into Xenopus oocytes to study recombinant ion channels

Real-time PCR

Cell preparations

Primary sensory neurons from dorsal root ganglia

Cultured mammalian cells to study recombinant ion channels