Pharmacology & Physiology
BIOGRAPHICAL SKETCH
NAME: POSITION TITLE:
Natalia Shirokova, Ph.D. Associate Professor
INSTITUTION AND LOCATION DEGREE YEAR(S) FIELD OF STUDY
MPhTI, Moscow, USSR MS 1987 Molecular/Chem. Phys.
Bogomoletz Institute of Physiology, PhD 1991 Physiology & Biophysics
Kiev, Ukraine
Rush University Medical School, Post-Doc 1991-96 Physiology
Chicago, USA
A. Positions and Honors
POSITIONS HELD
1987-1990: Aspirant MPhTI in the Bogomoletz Institute of Physiology, Kiev, Ukraine
(Advisors: Drs. I. Magura and P. Kostyuk).
1990-1997: Research Associate of the Department of General Physiology of the Nervous
System, Bogomoletz Institute of Physiology, Kiev, Ukraine.
1991-1996: Research Associate in the Department Molecular Biophysics and Physiology,
Rush University, Chicago, IL, USA .
1996-2000: Assistant Professor (non-tenure) in the Department Molecular Biophysics
and Physiology, Rush University, Chicago, IL, USA .
2000-date: Assistant Professor in the Department of Pharmacology and Physiology,
UMDNJ-New Jersey Medical School, Newark, NJ, USA.
HONORS
1985 MS with Honor, MPhTI.
1997-1998 Fellowship under the NIH Training Program
PROFESSIONAL MEMBERSHIPS
1991-date Biophysical Society.
1994-date Society of General Physiologists
REFERRAL WORK
American Journal of Physiology (Cell Physiol.), Biophysical Journal, Journal of Physiology
(London), Journal of General Physiology, Cell Calcium, Journal of Biological Chemistry,
European Heart Journal
B. Selected Peer-Reviewed Publications.
N.D. Ullrich, M. Fanchaouy, K. Gusev, N. Shirokova and E. Niggli. (2009). Hypersensitivity
of excitation-contraction coupling in dystropic mdx mouse cardiomyocytes. American
Journal of Physiology*****.
M. Fanchaou, E. Polakova, C. Jung, J. Ogrodnik, N. Shirokova and E. Niggli. (2009). Pathways
of abnormal stress-induced Ca2+ influx into dystrophic mdx cardiomyocytes. Cell Calcium,
46:114-121.
V.M. Shkryl, A.S. Martins, N.D. Ullrich, M.C. Nowycky, E. Niggli and N. Shirokova. (2009).
Reciprocal amplification of ROS and Ca2+ signals in stressed mdx dystrophic seletal muscle
fibers. Pflugers Archive, 458:915-928.
N. Shirokova and E. Niggli. Studies of RyR functions in situ. (2008). Methods, 46: 183-193.
W.J. Durham, P. Aracena-Parks, C. Long, A.E. Rossi, S.A. Goonasekera, S. Boncompagni,
D.L. Galvan, C.P. Gilman, M. Baker, N. Shirokova, F. Protasi, R. Dirksen and
S.L. Hamilton. (2008). RyR1 S -Nitrosylation underlies environmental heat stroke and
sudden death in Y522S RyR1 knock-in mice. Cell, 133: 53-65.
C. Jung, A.S. Martins, E. Niggli and N. Shirokova. Dystrophic cardiomyopathy: amplification
of cellular damage by Ca2+ signalling and reactive oxygen species-generating
pathways (2008). Cardiovascular Research, 77: 766-773.
A.S. Martins, V.M. Shkryl, M.C. Nowycky and N. Shirokova. Reactive oxygen species
contribute to Ca2+ signals produced by osmotic stress in mouse skeletal muscle
fibres (2008). Journal of Physiology, 586: 197-210.
E. Niggli and N. Shirokova. (2007). A guide to sparkology: The taxonomy of elementary
cellular Ca2+ signaling events. Cell Calcium, 42 ( 4-5 ): 379-387 .
E.V. Isaeva, V.M. Shkryl and N. Shirokova. (2006). Ca2+ sparks: SOS signals of
struggling muscle. Physiology News, 62: 27-28.
V.M. Shkryl and N. Shirokova. (2006). Transfer and tunneling of Ca2+ from sarcoplasmic
reticulum to mitochondria in skeletal muscle. Journal of Biological Chemistry,
281(3): 1547-1554.
E.V. I saeva, V.M. Shkryl and N. Shirokova. (2005). Mitochondrial redox state and Ca2+
sparks in permeabilized mammalian skeletal muscle. Journal of Physiology , 565: 855-872.
E.V. Isaeva and N. Shirokova. (2003). Metabolic regulation of Ca2+ release in
permeabilized mammalian skeletal muscle fibres. Journal of Physiology, 547: 453-462.
E. Ríos, N. Shirokova, W.G. Kirsch, G. Pizarro, M.D. Stern, H. Cheng and
A. González (2001). A Preferred Amplitude of Calcium Sparks in Skeletal Muscle.
Biophysical Journal, 80: 169-183.
G.A. Brum, A. González, J. Rengifo, N. Shirokova and E. Ríos. (2000). Fast imaging in
two dimensions resolves extensive sources of Ca2+ sparks in frog skeletal muscle.
Journal of Physiology, 528: 419-433.
A. González, W.G. Kirsch, N. Shirokova, G. Pizarro, G. Brum, I.N. Pessah, M.D. Stern, H.
Cheng and E. Ríos. (2000). Involvement of multiple intracellular release channels in calcium
sparks of muscle. Proceedings of National Academy of Science, 97: 4380-4385.
A. González, W.G. Kirsch, N. Shirokova, G. Pizarro, M.D. Stern and E. Ríos.(2000).
The spark and its ember. Separately gated local components of Ca2+ release in
skeletal muscle. Journal of General Physiology, 115: 139-157.
N. Shirokova, R. Shirokov, D. Rossi, A. González, W.G.Kirsch, J. García, V. Sorrentino
and E. Ríos. (1999). Spatially segregated control of Ca release in developing skeletal
muscle. Journal of Physiology, 521: 483-495.
E. Ríos, M.D. Stern, A. González, G. Pizarro and N. Shirokova. (1999). Release flux
underlying Ca2+ sparks in frog skeletal muscle. Journal of General Physiology 114: 31-48.
N. Shirokova, A. González, W.G. Kirsch, E. Ríos, G. Pizarro, M.D. Stern and H. Cheng.
(1999). Calcium sparks: release packets of uncertain origin and fundamental role.
Journal of General Physiology, 113: 377-384.
H. Cheng, L.S. Song, N. Shirokova, A. González, E.G. Lakatta, E. Ríos and M.D. Stern.
(1999). Amplitude distribution of calcium sparks in confocal Images. Theory and studies
with an automatic detection method. Biophysical Journal, 76: 606-17.
N. Shirokova, J. García and E. Ríos. (1998). Local calcium release in mammalian
skeletal muscle. Journal of Physiology, 512: 377-384.
N. Shirokova and E. Ríos. (1997). Small event Ca2+ release. A probable precursor of
Ca 2+ sparks in frog skeletal muscle. Journal of Physiology, 501: 3-11.
G. Pizarro, N. Shirokova, A. Tsugorka and E. Ríos. (1997). Quantal release of calcium
in skeletal muscle. Journal of Physiology, 501: 289-303.
N. Shirokova and E. Ríos. (1996). Activation of Ca2+ release by caffeine and voltage in
frog skeletal muscle. Journal of Physiology, 493: 317-339.
N. Shirokova and E. Ríos. (1996). Caffeine enhances intramembranous charge
movement in skeletal muscle by increasing cytoplasmic Ca 2+ concentration.
Journal of Physiology, 493: 341-356.
N. Shirokova , J. García, G. Pizarro and E. Ríos. (1996). Ca2+ release from the
sarcoplasmic reticulum compared in amphibian and mammalian skeletal muscle.
Journal of General Physiology, 107: 1-18.
N. Shirokova , A. González, J. Ma, R. Shirokov and E. Ríos. (1995). Properties and
roles of an intramembrane charge mobilized at high voltages in skeletal muscle.
Journal of Physiology, 486: 385-400.
N. Shirokova, G. Pizarro, and E. Ríos. (1994). A damped oscillation in the intramembrane
charge movement and calcium release flux of frog skeletal muscle fibers. Journal of
General Physiology, 104: 449-477.
R. Shirokov, R. Levis, N. Shirokova and E. Ríos. (1993). Calcium dependent inactivation
of cardiac L-type calcium channels does not involve their voltage sensor. Journal of
General Physiology, 102: 1005-1030.
R. Shirokov, R. Levis, N. Shirokova and E. Ríos. (1992). Two classes of gating current
from L-type Ca channels in guinea pig ventricular myocyte. Journal of General Physiology,
99: 863-895.
C. Research Support
Ongoing Research Support
NIH AR053933 �Mitochondria and Calcium Signaling in Skeletal Muscle",
PI, 09/19/08 - 08/31/13
American Heart Association, "Cardiac dystrophy: cellular mechanisms",
PI, 07/01/08 - 06/30/11
Muscular Dystrophy Association, "Calcium Signaling in Dystrophic heart",
PI, 01/01/07 - 03/01/09
Swiss Muscle Foundation Research, "Duchenne Muscular Dystrophy:
Pathophysiological Implications of mitochondrial
Calcium Signaling and ROS production". Co-PI, 01/01/06-12/31/08
Completed Research Support
R01 AR45690 NIH NIAMS, PI, 08/01/1999 - 07/31/2006
"Release Channel Isoforms and Local Calcium Signaling".
The major goal is to dissect the role of various isoforms of Ryr Ca2+
release channel in local Ca2+ signaling in muscle.
Muscular Dystrophy Association, PI, 07/01/02 - 06/30/05
�Metabolic Control of Calcium Signaling in Skeletal Muscle."