Peptide HPLC: Analytical/Without Fraction Collection

Automated peptide mapping by C-18 reverse-phase HPLC is available on a DX 500 (DIONEX) system. Digests are loaded in 50 µl of a suitable solvent, (which should not contain detergent) and should contain from 5 to 500 pmol protein. Unless otherwise requested, peptides will be detected by their absorbance at 220 nm.

 

Peptide HPLC: Preparative/With Fraction Collection

Protein digests that contain from 5 to 500 picomoles in a volume of 50 µl may be fractionated by C-18 reverse-phase HPLC collected fractions . If further purification is required, individual peaks collected from an HPLC collection run of a protein digest can be re-chromatographed on C-18 reverse phase.