Q. How much DNA do I need per reaction?

Template:

Plasmid (3-6 kB) : 0.5-1.0 ug. or Plasmid (6-12 kB) : 1.0-1.5ug. or Plasmid (> 12 kB): Contact us.

PCR fragment: 10 ng per 100 bp (ex. 400 bp = 40 ng).

Primer: 20ng/ul or 3 pmols/ul.

Q. How close to the primer can I read?

Readable sequence usually begins 30-50 bases after the primer.

Q. Why was my sample blank?

This is our most common question and the most difficult to answer since a truly blank sample provides no information about what went wrong. Listed below are the most common reasons for blank samples:

1. Low template concentration. 2. Incorrect primer used or no primer annealing site in the template. 3. Impurities in the template prep or primer. 4. Extremely high GC content or template secondary structure.

Q. Turn-around time?

12 pm to 10 am (20 hr.)

Q. How far will I be able to read my sequence?

The overall sequence quality is, of course, very dependent on DNA quality and signal strength. A typical run generates about 1000 bases with an accuracy of 98-99% for the first 700 to 800 bases. As you read past 600 bases the accuracy decreases. High quality samples often generate accurate sequence to 750 bases and beyond.

Q. Is there any discount for bulk orders?

Yes. More than 100 samples in a month. Inquire about the price.

Q. What are the facility supplied primers?

M13 F, M13 R, SP6 promoter, T7 promoter, T7 terminator, T3 promoter, BGH reverse, pGEX 5', pGEX 3', GLprimer 1, GLprimer 2, RVprimer 3, RV primer 4 . (free of cost).