The facility houses two Real Time PCR systems.

The LightCycler® 2.0 Instrument (Roche) allows you to monitor amplification of a PCR product simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.05 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. 
The amplification reaction occurs in specially designed glass capillaries. Their superior surface-to-volume ratio guarantees extremely rapid thermal transfer, which facilitates high-speed thermal cycling.
The combination of glass capillaries and the air-driven temperature control of the LightCycler® 2.0 Instrument ensures rapid PCR. An entire 35-cycle run can be performed in as little as 30 minutes (with 20 µl capillaries) to 60 minutes (with 100 µl capillaries).
Fluorescence detection formats: The LightCycler® 2.0 Instrument is optimized for two fluorescence detection formats: SYBR Green I and HybProbe probes. In addition, the instrument supports a wide variety of other fluorescence detection formats, such as monocolor SimpleProbe probes, hydrolysis probes, and other formats based on FRET (fluorescence resonance energy transfer). However, it is essential that the fluorescent dyes used are compatible with the LightCycler® 2.0 optical unit.
Software: The LightCycler® 2.0 Instrument utilizes the LightCycler® Software 4.05, an optimized version of the LightCycler® 4.0 Software, which provides advanced data-analysis modules, efficient data management, and effective data protection.

The Applied Biosystems 7500 Real Time PCR Systems uses fluorescent-based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and association-curve analysis.

Run time of less than 2 hours.

Variable excitation and five-color detection enable you to use a broad range of fluorophores including FAM™/SYBR® Green I, VIC®/JOE, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes for greater assay versatility.

Measure gene expression levels , detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.

Supports 96-well format plates and 0.2 mL tubes.

Powerful, versatile software including plate set-up wizards and automated analysis tools make data processing simple and straightforward.

 

Reagents for both instruments are available through the supply center.

For any questions please contact the DNA Sequencing lab by calling (973) - 972 - 2625 or by emailing mrfadm@umdnj.edu