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> Confocal Imaging Facility
About our Facility
Welcome to the Confocal
Imaging Facility, a core facility of NJMS-UMDNJ.
We provide advanced microscope systems for optical imaging of live and fixed
samples. Facility Manager Peter Carroll
is trained in cell biology, has many years' experience
in 3D microscopy, and specializes in working with users to find the best methods
for any imaging study and assisting users as needed with experimental design,
image acquisition, training, technical support and data interpretation. He is
on site every weekday to facilitate imaging and in-depth consultation. Please
feel free to stop by anytime!
Scanning
Confocal Microscopes:
The Facility has two scanning Confocal
systems, all attached to inverted microscopes. Each of these systems has unique
imaging capabilities beyond those of the traditional confocal microscope, but
samples must be prepared on sealed slides, chamber slides or chamber/dish with
coverslip bottom.
Zeiss LSM 510 on
Zeiss Axiovert 100M Base:
 Zeiss
LSM 410 on Zeiss Axiovert 100 Base:
- Has an argon laser
(488, 568, 647 nm)
- Allows for simultaneous collection of two fluorescence signals and a visible
image.
 Zeiss
PALM Laser-Capture System on
Zeiss Axiovert 200M Base:
- DNA, RNA and protein isolation from slides or live cells
- Automated image analysis in transmitted light and fluorescence (FITC, Rhodamine,
Alexa Fluor 430)
Data
Management:
All systems record images digitally,
and all resulting images are viewable with both commercial and free software
on Macintosh, Windows and UNIX/Linux operating systems.
Data is placed on a
server for convenient download in your laboratory; files can also be written
to DVD, CD and portable flash drives.
All images are compatible
with software such as Photoshop and PowerPoint for publications and presentations
Software
Capabilities:
The staff provides consultation and
training on the use of various software packages for imaging enhancement and
analysis. Some examples of what you can do are:
• Pseudocolor, background
correction, image enhancement, ratio imaging
• 3D rendering of data in
stereo or rotating projections
• Movie creation for time-lapse
experiments
• 2D particle tracking
• Morphometry (object length,
volume, etc)
• Quantitative intensity
measurements
• Colocalization
Contact
Us:
Please feel free to stop by the Facility
anytime, or contact us via phone or e-mail to discuss your experiments and to
see how we can help you in your imaging. Our contact information is:
Manager: Peter Carroll
NJMS Cancer Center, G-1105
Phone: (973) 972-8101
E-mail: carrolpb@umdnj.edu
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